paraformaldehyde solution (Thermo Fisher)

Thermo Fisher is a verified supplier

Thermo Fisher manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Thermo Fisher paraformaldehyde solution
Paraformaldehyde Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fixative+solution/Paraformaldehyde+fixative+solution/pm42331167-97-6-38
Average 97 stars, based on 1 article reviews

paraformaldehyde solution - by Bioz Stars, 2026-10

97/100 stars

Images

Infection:

Article Title: Rational Design of a Chimpanzee Adenoviral-Vector Vaccine Against Yellow Fever Through the Modification of Antigen Transmembrane Domains.
Article Snippet: .. T-RexTM-293 cells (Thermo Fisher Scientific, UK) grown on glass coverslips of 13 mm diameter were infected at a MOI of 10 IU/cell with ChAdOx1 viruses and incubated at 37 ◦C under 5% CO2 for 18 h. After 18 h, the cells were fixed with Image-ITTM Paraformaldehyde 4% Fixative solution (Thermo Scientific, UK) for 10 min at room temperature (RT), followed by permeabilization with a PBS buffer containing 0.1% v/v Triton-X100 in PBS. .. The YFV E proteins were visualized using the Yellow Fever Virus antibody 2D12 (0G5) (Bio-Rad, Watford, UK), and the Golgi apparatus was visualized using the Anti-GM130 antibody [EP892Y] cis-Golgi Marker (Abcam, Cambridge, UK), both at dilutions of 1:100 in a PBS buffer containing 2% v/v FBS (blocking buffer), for 1 h at RT.

Incubation:

Article Title: Programmed DNA elimination was present in the last common ancestor of Caenorhabditis nematodes
Article Snippet: .. Worms were then dissected in 10 μl of 1 mM levamisole on poly-L-lysine-coated slides, freeze-cracked, and fixed in pre-chilled methanol (−30°C) in a fixation jar for 10 min. Each slide was washed twice in PBST (0.05% Tween-20 in 1X PBS buffer), followed by incubation with 40 μl of fixative solution (3.7% formaldehyde, 0.8% methanol in 1X PBS) for 10 min. After a PBST wash, slides were treated with 2X SSCT (2X saline-sodium citrate [SSC] buffer [Thermo Fisher Scientific: 15557–04] and 0.5% Triton X-100). .. They were incubated in denaturation buffer (50% formamide in 1X SSCT) at 60°C for 30 min, then overlaid with 10 μl of probe mixture (10% dextran sulfate, 2X SSC, 50% formamide, 1 μM of DNA probe [Ca_SR2: 5’-Alexa488-GTGGACAAAAATCGACAAAACAAAGACC-3’ (Eurofins), Ca_SR8: 5’-CY3-CAGTTTTGACGTCATCAGTTCAA-3’ (Eurofins), Telomere: 5’-CY3-TTAGGCTTAGGCTTAGGCTTAGGC-CY3-3’, (Integrated DNA Technologies)], 1 mg/ml of RNase A and 0.1% Tween-20).

Saline:

Staining:

Article Title: Surface‐Capped Protein Nanoparticles for Nonviral Gene Delivery
Article Snippet: .. After fixation, the fixative solution was discarded, and the cells were washed gently with PBS 3 times, followed by permeabilization with 0.25% Triton‐X100 (in PBS) for 10 min. After the permeabilization solution was discarded, and the cells were washed 3 times with PBS, Alexa Fluor 488 Phalloidin (1:400, Invitrogen) solution was added to each well to stain the cytoskeleton. .. To visualize the cell nuclei, scaffolds were stained with DAPI staining solution (1:1000, Abcam) for 10 min at RT.

Review